Allostery in the nitric oxide dioxygenase mechanism of flavohemoglobin
AM Gardner, PR Gardner - Journal of Biological Chemistry, 2021 - ASBMB
… activate the NO dioxygenase function of Escherichia coli flavohemoglobin. Steady-state and
… E and F-helices and switches an electron transfer gate formed by LysF7, GlnE7, and water. …
… E and F-helices and switches an electron transfer gate formed by LysF7, GlnE7, and water. …
[HTML][HTML] Flavohaemoglobin: The pre-eminent nitric oxide–detoxifying machine of microorganisms
RK Poole - F1000Research, 2020 - ncbi.nlm.nih.gov
… It is soluble in water (approximately 1.6 mM at 37 C and 1.94 … to the NO dioxygenase
activities of flavohaemoglobin 12 . … Thus, for example, the Escherichia coli flavohaemoglobin (…
activities of flavohaemoglobin 12 . … Thus, for example, the Escherichia coli flavohaemoglobin (…
A nitric oxide synthase–like protein from Synechococcus produces NO/NO3− from l-arginine and NAPDH in a tetrahydrobiopterin-and Ca2+-dependent manner
AL Picciano, BR Crane - Journal of Biological Chemistry, 2019 - ASBMB
… Escherichia coli strains against nitrosative stress, even in a mutant devoid of NO-protective
flavohemoglobin… NOS and NO oxygenase activities, requires H 4 B, and may play a role in Ca …
flavohemoglobin… NOS and NO oxygenase activities, requires H 4 B, and may play a role in Ca …
Dioxygen and glucose force motion of the electron-transfer switch in the iron (III) flavohemoglobin-type nitric oxide dioxygenase
AM Gardner, PR Gardner - Journal of Inorganic Biochemistry, 2023 - Elsevier
… of the Escherichia coli flavoHb-NOD [8,9]. However, in the resting state, the Fe(III)-flavoHb
binds … and the bound ‘Coulomb blockade’ water molecule poised between the FAD C8M and …
binds … and the bound ‘Coulomb blockade’ water molecule poised between the FAD C8M and …
Ordered Motions in the Nitric-Oxide Dioxygenase Mechanism of Flavohemoglobin and Assorted Globins with Tightly Coupled Reductases
PR Gardner - Protein Reviews: Volume 23, 2022 - Springer
… -detoxifying NO metabolic activity with the flavoHb in Escherichia coli (Gardner et al. 1998a;
… modeled on the E. coli ferric flavoHb with a bridging Coulomb blockade water molecule (W) …
… modeled on the E. coli ferric flavoHb with a bridging Coulomb blockade water molecule (W) …
[HTML][HTML] A critical role for staphylococcal nitric oxide synthase in controlling flavohemoglobin toxicity
RM Singh, SS Chaudhari, S Panda, EH Hutfless… - Redox Biology, 2023 - Elsevier
… Under these conditions, growth can only resume once nitric oxide (NO) is detoxified by the
flavohemoglobin Hmp. Paradoxically, S. epidermidis produces endogenous NO through its …
flavohemoglobin Hmp. Paradoxically, S. epidermidis produces endogenous NO through its …
Molecular characterization and expression profiling of two flavohemoglobin genes play essential roles in dissolved oxygen and NO stress in Saitozyma podzolica …
Q Yang, Y Ran, S Guo, F Li, D Xiang, Y Cao… - International Journal of …, 2023 - Elsevier
… Hence, the Fhb, also known as NO-dioxygenase, can … in an ice bath and a boiling water bath
three times for 10 min. … washed twice with 1 mL of distilled water, and re-suspended in 1 mL …
three times for 10 min. … washed twice with 1 mL of distilled water, and re-suspended in 1 mL …
Impact of azole drugs on energetics, kinetics, and ligand migration pathways of CO photo-dissociation in bacterial flavohemoglobins
D Butcher, M Moussaoui, L Baciou, J Miksovska - RSC advances, 2020 - pubs.rsc.org
… fHbs exhibit a nitric oxide dioxygenase (NOD) activity which … of electron transfer in
flavohemoglobins, specifically disruption of the … groups through adjacent structural water …
flavohemoglobins, specifically disruption of the … groups through adjacent structural water …
Interdomain electron transfer in flavohaemoglobin from Candida norvegensis with antibiotic azole compounds
K Kobayashi, J Igarashi, T Kozawa - FEBS letters, 2022 - Wiley Online Library
… (FlavoHbs) function as nitric oxide dioxygenases, oxidizing … known as Alcaligenes eutrophus),
Escherichia coli [[6]] and … interacts with both the bridging water group and the propionate …
Escherichia coli [[6]] and … interacts with both the bridging water group and the propionate …
Transcriptomic Identification and Biochemical Characterization of HmpA, a Nitric Oxide Dioxygenase, Essential for Pathogenesis of Vibrio vulnificus
D Kim, EJ Na, S Kim, JS Kim, YH Jung, J Cao… - Frontiers in …, 2019 - frontiersin.org
… NO dioxygenases are a family of flavohemoglobins (Hmp) … identified hmpA, a homolog of
Escherichia coli hmp (Echmp), … solution and then degassed distilled water. The purified NO gas …
Escherichia coli hmp (Echmp), … solution and then degassed distilled water. The purified NO gas …